In 2D PAGE, the gel is setup with a pl that starts at 6.2 and increases to a pl

Question

In 2D PAGE, the gel is setup with a pl that starts at 6.2 and increases to a pl of 6.8. If you placed the proteins at the starting end, which proteins move the farthest? a. the largest b. the smallest c. the proteins with a net zero charge at pH 6.2 d. the proteins with a pl of 6.5 e. the proteins with a pl of 6.6 In the "sizing" part of 2D PAGE, which proteins move the farthest during polyacrylamide gel electrophoresis? a. the largest b. the smallest c. the most positive d. the proteins with a pl of 6.4 e. the proteins with a pl of 6.6

Explanation / Answer

53. Ans: e .The protein with a Pi 6.6 moves the farthest.

In Isoelectric focusing a steady state mechanism works with regards to Ph. Protein approach to their respective pI values at different rates. The strip protein has a range from 6.2 to 6.8. When electric passed the protein of PI 6.6 moves toward the 6.8 end and stop at 6.6 Pi.

54. Ans: b. the smallest protreins move the farthest during PAGE.

In PAGE protein are moving based on their size through the pores of gel. The smaller one moves fast and the larger one moves slowly.

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