I need help with this problem: 1. Add 0.0005 liters of water to 5 eppendorf tube

Question

I need help with this problem:

1. Add 0.0005 liters of water to 5 eppendorf tubes labeled 1-5.

2. Then add 0.0005 liters of 1 M HCL solution to the tube that is labeled 1. Mix well. Then make a 1:2 dilution with the next 4 tubes(the concentration of HCL becomes less and less). What is the concentration of HCL in these tubes?

3. Label 5 new eppendorf tubes A-E, and add 0.0009 liters of cell culture medium to each of the tubes.

4. From tube number 1, add 0.0001 liters of HCL solution to tube A. And then add 0.0001 liters of HCLsolution from tube number 2 to tube number B, and then the same from tube number 3 to tube number C, and from tube number 4 to tube number D, and from tube number 5 to tube number E. What is the concentration of HCL in the the 5 different tubes?(The cell culture medium has a pH between 7 and 8)

Explanation / Answer

HCl concentration

1 tube 0.5M

2 tube 0.25M

3 tube 0.125M

4tube 0.0625 M

5th tube 0.03125

HCL concentration

A 0.056M

B 0.028

C 0.0139

D 0.00694M

E 0.00347 M

pH is depend on HCl and also cell culture medium. In this question they didn't mentioned the pH of pure cell culture medium

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects