I am try to separate a mixture using high preformance liquid chromatography (HPL

Question

I am try to separate a mixture using high preformance liquid chromatography (HPLC) however my instrument doesn't have a fraction collector. How can I collect fractions before it goes to waste? Do I start collecting once I see it eluting on the computer screen (at its elution time) ? Or well before or after its elution time? Need to know the best method to use when a fraction collector is not available. I am try to separate a mixture using high preformance liquid chromatography (HPLC) however my instrument doesn't have a fraction collector. How can I collect fractions before it goes to waste? Do I start collecting once I see it eluting on the computer screen (at its elution time) ? Or well before or after its elution time? Need to know the best method to use when a fraction collector is not available. I am try to separate a mixture using high preformance liquid chromatography (HPLC) however my instrument doesn't have a fraction collector. How can I collect fractions before it goes to waste? Do I start collecting once I see it eluting on the computer screen (at its elution time) ? Or well before or after its elution time? Need to know the best method to use when a fraction collector is not available. I am try to separate a mixture using high preformance liquid chromatography (HPLC) however my instrument doesn't have a fraction collector. How can I collect fractions before it goes to waste? Do I start collecting once I see it eluting on the computer screen (at its elution time) ? Or well before or after its elution time? Need to know the best method to use when a fraction collector is not available.

Explanation / Answer

You can minimize the errors collecting effluent mobile phases as long as the peak appears, because the concentration after of it tends to change quickly.

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