In which you transferred 01 ml of your urine sample onto a plate, you counted 23
ID: 99969 • Letter: I
Question
In which you transferred 01 ml of your urine sample onto a plate, you counted 2370 colonies in your plate. Calculate the number of organisms per milliliter of sample. Is the above number significant in terms of disease? Use the picture below to answer the following questions? a- How many sections are streaked in this plate? b- Using an arrow indicate which section you would use to isolate pure cultures. c. In your opinion, would you say the student did a good job streaking the plate? True or False A Pure culture contains a single species of organism. What microorganism is the frequent cause of urinary tract infections seen in hospitals? In lab #6, why were you told to inoculate the TGYA shakes tubes, at a temperature higher than 45 degree C? What 2 enzymes does Clostridium lack that prevents it from growing in oxygen or makes the presence of oxygen lethal? What is the difference between the Dorner and Schaeffer-Fulton method of spore staining? Given the picture below, indicate with an arrow which antibiotics were least effective?Explanation / Answer
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A) There are 2370 colonies in 0.01 ml of urine sample of an individual. So, 1 ml of sample contains 237000 organisms that is the colony forming units.
So the 2,37,000 cful/ml is the count of the urine sample.
B) Diagnosis if Urinary tract infection is based in quantitative urine culture that yields 1,00,000 CFU/ml. And here we are getting 2,37,000Cfu/ml that means this is significant for the disease.