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Use the following information to answer the question(s) below. A transfer RNA (#

ID: 99981 • Letter: U

Question

Use the following information to answer the question(s) below. A transfer RNA (#1) attached to the amino acid lysine enters the ribosome. The lysine binds to the growing polypeptide on other tRNA (#2) already in the ribosome. Where does tRNA #2 move to after this bonding of lysine to the polypeptide? A) exit tunnel B) A site C) directly to the cytosol D) P site E) E site Which component of the complex described enters the exit tunnel through the large subunit of the ribosome? A) tRNA with polypeptide (#2) B) newly formed polypeptid e C) initiation and elongation factors D) tRNA with attached lysine (#1) E) tRNA that no longer has attached amino acid Which of the following is not true of a codon? A) It may code for the same amino acid as another codon. B) It is the basic unit of the genetic code. C) It extends from one end of a tRNA molecule. D) It never codes for more than one amino acid. The anticodon of a particular tRNA molecule is A) complementary to the corresponding mRNA codon. B) complementary to the corresponding triplet in rRNA. C) catalytic, making the tRNA a ribozyme. D) the part of tRNA that bonds to a specific amino acid. What component is not involved in translation? A) ribosomes B) #RNA C) DNA D) GTP

Explanation / Answer

56) e – E site

“E” site is the site for exit of the tRNA 2 (tRNA that has no amino acid attached to it) after binding with lysine for the formation of the polypeptide. During translation, after the peptide bond is formed the mRNA is pulled through the ribosome and the tRNA moves out through the E (exit) site.

57) b – newly formed polypeptide.

Elongation stops when there is stop codon. “A” site will not accept anymore tRNA and then releasing factor binds to “A” site. This releasing factor breaks the bond between tRNA and polypeptide and releases polypeptide which moves through the exit tunnel of larger ribosomal subunit. Polypeptide undergoes conformational changes while it passes through the exit tunnel.

58) c – It extends from one of the tRNA molecule.

Codon is a set of three adjacent nucleotides on mRNA which codes for an aminoacid.

59) a - complementary to the corresponding mRNA codon.

Anticodon of tRNA is sequence containing three bases.

60) c - DNA

The different components involved in translation are mRNA, tRNA, ribosomes, protein factors and biochemical energy (in the form of ATP, GTP).