The following statements describe SDS-PAGE, a method used to separate proteins.
ID: 1009327 • Letter: T
Question
The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements
The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements. Assume that SDS-PAGE is performed under reducing conditions. Protein-SDS complexes have similar mass to charge ratios, therefore separation is by size. Smaller proteins migrate faster through the polyacrylamide gel. Sodium dodecyl sulfate binds proteins, resulting in protein-SDS complexes that are similar in size. Proteins are separated in a polyacrylamide gel matrix. Protein-SDS complexes migrate toward the negative electrode. Proteins are visualized using a dye that binds to the gel matrix, but not to proteins.Explanation / Answer
True statements,
1.smaller protein migrate faster through tha polyacrymide.
2. proteeins are seperated in a polyacrcylamide gelmatrix.
(SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) is commonly used in the lab for the separation of proteins based on their molecular weight. It’s one of those techniques that is commonly used but not frequently fully understood. So let’s try and fix that.
SDS-PAGE separates proteins according to their molecular weight, based on their differential rates of migration through a sieving matrix (a gel) under the influence of an applied electrical field.)