The following statements describe SDS-PAGE, a method used to separate proteins.
ID: 478643 • Letter: T
Question
The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements. Assume that SDS-PAGE is preformed under reducing conditons.
SDS-PAGE utilizes agarose gel to separate proteins. Sodium dodecyl sulfate binds proteins, resulting in protein-SDS complexes that are similar in size. A protein binds roughly 1.4 times its mass of SDS resulting in a large overall negative charge. Smaller proteins migrate faster through the polyacrylamide gel. Proteins are visualized using a dye that binds to the gel matrix, but not to proteins M Protein-SDS complexes migrate toward the positive electrodeExplanation / Answer
1)SDS-PAGE utilizes agarose gel to seperate protines
2) smaller protines migrate father than larger protines