The following statements describe SDS-PAGE, a method used to separate proteins.
ID: 273426 • Letter: T
Question
The following statements describe SDS-PAGE, a method used to separate proteins. Some of the statements are correct, and some of them are incorrect. Select all the correct statements.
The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements. Assume that SDS-PAGE is performed under reducing conditions. Sodium dodecyl sulfate binds proteins, resulting in protein-SDS complexes that are similar in size. Proteins are separated in a polyacrylamide gel matrix. teins are visualized using a dye that binds to the gel matrix, but not to proteins. Protein-SDS complexes migrate toward the negative electrode. Smaller proteins migrate faster through the polyacrylamide gel. Protein-SDS complexes have similar mass to charge ratios, therefore separation is by size. ?Explanation / Answer
Ans-
Proteins are separated in a polyacrylamide gel matrix
Smaller proteins migrate faster through the polyacrylamide gel
Protein-SDS complexes have a similar mass to charge ratios, therefore separation is by size
Explanation:
PAGE or PolyAcrylamide Gel Electrophoresis is a semi-solid phase that used to separate protein through electrophoresis according to their size. The separation of proteins is based on the charged molecules that migrate through a matrix by application of an electrical field. When proteins are separated in a gel matrix by electrophoresis, smaller proteins migrate faster because of less resistance from the gel matrix
SDS, a polyanionic detergent is used to denature the protein structure. All the protein samples run on the gel is negatively charged, so moves towards the positive electrode. Also, all the samples have a similar mass to charge ratios, so the proteins are separated only according to their molecular weight during electrophoretic separation.