The following statements describe SDS-PAGE, a method used to separate proteins.
ID: 203509 • Letter: T
Question
The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements. Assume that SDS-PAGE is performed under reducing conditions. Smaller proteins migrate faster through the polyacrylamide gel. Protein-SDS complexes migrate toward the positive electrode. SDS-PAGE utilizes agarose gel to separate proteins. Proteins are visualized using a dye that binds to the gel matrix, but not to proteins. Sodium dodecyl sulfate binds proteins, resulting in protein-SDS complexes that are similar in size. Protein-SDS complexes have similar mass to charge ratios, therefore separation is by size.Explanation / Answer
True statement on SDS PAGE
Smaller proteins migrate faster through the polyacrylamide gel
Protein-SDS complexes migrate toward the positive electrode
Protein-SDS complexes have similar mass to charge ratios, therefore separation is by size