There are three stations set up around the lab, one for proteins, one for starch
ID: 101150 • Letter: T
Question
There are three stations set up around the lab, one for proteins, one for starch, and one for fats. At each station, there are sets of four test tubes containing the particular biomolecule type for that station. In addition, each of the four test tubes at each station also contains one of the following enzymes or control: i) Control (no enzyme). ii) lipase, iii) amylase, iv) pepsin (protease) The enzyme (or control) in each of the lettered test tubes is the same for all of the stations. The test tubes sat with the enzymes (or control) for 24 hours, and then each tube was treated with a reagent that produces a positive test color in the presence of the macromolecule. Table 3: Color Test For Molecules using Test Reagents. When no color was produced, the test was negative because the molecule was not present. These negative results are left blank in the table. A positive test showed a color because the molecule was present. (Students: Do not fill in this table. It is complete.)Explanation / Answer
Control treatment (Pepsin): Proteins or peptides when treated with peosin in acuduc environment and incubated at 37 degrees overnight, it results in the formation of amino acids. The presence of amino acids can be tested by Ninhydrin test which changes the color of the solution to violet or purple. Biuret test also gives violet color.
Experimental treatment 1:
Starch: Starch when treated with amylase and incubated at 37 degrees temperature overnight produces glucose. The presence of glucose can be demonstrated by Benedict's test which produces red-orange color. The presence of starch is identified by Lugol's test which produces blue-black color.
Experimental treatment 2:
Fats: Fats on treatment with lipase produces fatty acids which reacts with Sudan IV dye to produce red color.