Place the stapamein lyisin the coe Calculate the Rf value for each compound. Use

Question

Place the stapamein lyisin the coe Calculate the Rf value for each compound. Use tweezers to remove the plate and immediately mark the solvent front. Mark the spot(s) due to the compound(s). This might require a visualization technique. Lightly mark the edges of the TLC plate at the origin line with a pencil. Develop the chromatogram until the front of the mobile phase is 1-1.5 cm from the top of the plate. Using tweezers, carefully place the spotted TLC plate into the developing chamber. Obtain a pre-coated TLC plate and a TLC developing chamber Spot the TLC plate with a dilute solution (1-2%) of the compounds to be analyzed. Prepare the developing chamber with the mobile phase

Explanation / Answer

Obtain a precoated TLC plate and developing chamber

Prepare the developing chamber with mobile phase

Lightly mark the edges of the TLC plate at the origin line with a pencil

Spot the TLC plate with a dilute solution of the compounds to be analysed

Using tweezers carefully place the spotted TLC plate into the developing chamber

Develop the chromatogram until the front of the mobile phase is 1-1.5 cm from the top of the plate

Use tweezers to remove the plate and immediately mark the solvent front

Mark the spots due to the compound. This might require a visualization technique

Calculate Rf value of each compound

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