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Map How will the following changes affect the retention time of the solute in su

ID: 1040989 • Letter: M

Question

Map How will the following changes affect the retention time of the solute in supercritical fluid chromatography ienwupercfriloaina ton cioxide is used as the monie chromatography Increase the pressure while maintaining a constant temperature and flow rate O unchanged decrease O not enough information O increase Decrease the temperature while maintaining a constant pressure and flow rate O unchanged O increase not enough information decrease Increase the flow rate while maintaining a constant pressure and temperature O increase not enough information decrease O unchanged

Explanation / Answer

Increase in the pressure decreases the retention time as the density increases.

Decrease the temperature decreases the retention time, as the number of theoretical plates in the column is reduced slightly.  

Increase the flow rate decrease the retention time as the pressure increases.