Map Sapling Learning macrmilanlaamino The following questions apply to separatio
ID: 1064648 • Letter: M
Question
Map Sapling Learning macrmilanlaamino The following questions apply to separation of a solution containing pentanol (FM 88.15) and 2,3-dimethyl-2- butanol (FM 102.17). Pentanol is the internal standard. (a) Separation of a standard solution containing 209 mg of pentanol and 237 mg of 2,3-dimethyl-2-butanol in 10.0 mL of solution led to a pentanol:2,3-dimethyl-2-butanol relative peak area ratio of 0.882:1.00. Calculate the response factor, F, for 2,3-dimethyl-2-butanol. Number b) Calculate the areas for pentanol and 2,3-dimethyl-2-butanol gas chromatogram peaks in an unknown. or pentanol, the peak height was 79.9 mm and the width at half-height was 2.7 mm. For 2,3-dimethyl-2- butanol, the peak height was 53.5 mm and the width at halfheight was 4.8 mm. Assume each peak to be a Gaussian Number Pentanol Number 2,3-Dimethyl-2-butanol O mm (Scroll down for more questions.)Explanation / Answer
From the separation data
(a) Response factor for 2,3-dimethyl-2-butanol = (1/237)/(0.882/209)
= 1.0
(a) area for peak = height x (width/2)
area for 2,3-dimethyl-2-butanol = 53.5 x 4.8 = 256.8 mm^2
area for pentanol = 79.9 x 2.7 = 215.73 mm^2