In the selection of a detector for a chromatographic method utilizing a gradient
ID: 1072590 • Letter: I
Question
In the selection of a detector for a chromatographic method utilizing a gradient, some possible choices would be (a) ultraviolet detector, refractive index detector, or an electrochemical detector. (b) ultraviolet detector, fluorescence detector, or mass spectrometer. (c) refractive index detector, electrochemical, detector, and conductivity detector. Which terms/items are associated with HPLC? (a) Reversed phase, air blank, and sample loop. (b) Reversed phase, polymeric C18 bonded phase, and thermal conductivity detector. (c) Reversed phase, polymeric C18 bonded phase, and sample loop. Which statement is correct? (a) A HPLC solvent system that begins with 20:80 acetonitrile:water and ends with 50:50 acetonitrile:water is gradient elution. (b) A HPLC solvent system that begins with 20:80 acetonitrile:water and ends with 50:50 acetonitrile:water is an isocratic elution. (c) A HPLC solvent system that begins with 20:80 acetonitrile:water and ends with 50:50 acetonitrile:water is an isosolvent elution.Explanation / Answer
10. In the selection of a detector for gradient run,
(a) ultraviolet detector, refractive index detector or an electrochemical detector
11. Terms associated with HPLC,
(c) reversed phase, C-18 bonded phase and sample loop
12. True statement would be,
(a) a HPLC begining with 20 : 80 CH3CN : H2O and ending in 50 : 50 CH3CN : H2O is gradient elution.