An enzyme was reacted with a site specific inhibitor for a few minutes and then

Question

An enzyme was reacted with a site specific inhibitor for a few minutes and then assayed for activity. The Vmax of the enzyme was reduced from 100 umol/min to 20 umol/min. The inhibitory reaction was repeated using the same amount of inhibitor under the same conditions, except that a 5 mM concentration of the enzyme's substrate was present in the reaction mixture. Under these revised conditions the Vmax of the enzyme was reduced to 50 umol/min. Offer some explanations as to why there was less inhibition when the enzyme's substrate was present in the reaction mixture. 8.

Explanation / Answer

Vmax reflects how fast the enzyme can catalyze the reaction.So when inhibitor was present enzyme efficiency gats reduced to 100 mol/min to 20 mol/min.But when substrate was present, it will compete enzyme and therefore,Vmax turn 50 mol/min comparatively higher than above with inhibitor.

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