Please answer the 3 following question and provide a short reason why you chose
ID: 11344 • Letter: P
Question
Please answer the 3 following question and provide a short reason why you chose your answer.
34. Which of the following techniques would be useful for determining whether Tst1, a transmembrane
protein, localizes to a particular domain of the cellular plasma membrane?
A) freeze fracture
B) immuno electron microscopy
C) bright field microscopy
D) confocal microscopy
E) GFP labeling followed by fluorescence microscopy
F) GFP labeling followed by electron microscopy
35. Phospholipid molecules tend to spontaneously form bilayers when dispersed with water. In contrast,
detergent molecules are more prone to form spherical micelles. Which of the following properties of
phospholipids is most important in promoting bilayer formation over micelle formation?
A) Phospholipids have a polar or charged head group whereas detergents do not.
B) Phospholipids have a higher degree of saturation in their fatty acid tails compared to detergents.
C) Phospholipids have longer fatty acid tails than detergents and this prevents micelle formation.
D) Phospholipids found in membranes have two fatty acid tails and are shaped like cylinders. Thus, their
optimal packing arrangement is to form a bilayer rather than a cone shaped micelle.
E) B and C are important.
F) A, B and C are important
.
36. Which of the following would have to be true if H+ was to be used to drive co-transport of an amino
acid into a cell or organelle? Note that a high concentration of the amino acid is already present in the cell
or organelle. (circle all that are true)
A) A channel for the amino acid would have to be present on the membrane.
B) A binding site for H+ must be present on the carrier.
C) There would have to be a H+ channel on the membrane.
D) A binding site for the amino acid would have to be present on the carrier.
E) The membrane would have to contain a carrier that could actively transport H+.
F) The membrane would have to allow H+ to diffuse across.
Explanation / Answer
Please answer the 3 following question and provide a short reason why you chose your answer.
34. Which of the following techniques would be useful for determining whether Tst1, a transmembrane
protein, localizes to a particular domain of the cellular plasma membrane?
A) freeze fracture
B) immuno electron microscopy
C) bright field microscopy
D) confocal microscopy
E) GFP labeling followed by fluorescence microscopy
F) GFP labeling followed by electron microscopy
35. Phospholipid molecules tend to spontaneously form bilayers when dispersed with water. In contrast,
detergent molecules are more prone to form spherical micelles. Which of the following properties of
phospholipids is most important in promoting bilayer formation over micelle formation?
A) Phospholipids have a polar or charged head group whereas detergents do not.
B) Phospholipids have a higher degree of saturation in their fatty acid tails compared to detergents.
C) Phospholipids have longer fatty acid tails than detergents and this prevents micelle formation.
D) Phospholipids found in membranes have two fatty acid tails and are shaped like cylinders. Thus, their
optimal packing arrangement is to form a bilayer rather than a cone shaped micelle.
E) B and C are important.
F) A, B and C are important
.
36. Which of the following would have to be true if H+ was to be used to drive co-transport of an amino
acid into a cell or organelle? Note that a high concentration of the amino acid is already present in the cell
or organelle. (circle all that are true)
A) A channel for the amino acid would have to be present on the membrane.
B) A binding site for H+ must be present on the carrier.
C) There would have to be a H+ channel on the membrane.
D) A binding site for the amino acid would have to be present on the carrier.
E) The membrane would have to contain a carrier that could actively transport H+.
F) The membrane would have to allow H+ to diffuse across.