Please explain answers and show formulas used and label units Fluorescent molecu

Question

Please explain answers and show formulas used and label units

Fluorescent molecules can be used to track biomolecular events one molecule at a time.
Fluorescence is very similar to the atomic transitions studied in lecture. Consider a
fluorescent molecule where the electron that participates in fluorescence has ground state energy of
-4.6 eV. When the molecule is illuminated with light this electron is excited up to a state
with energy -2.3 eV.

4) (2 pt) Now consider the protein-ligand system shown in the figure. When the molecules
are separated the fluorescent molecule is exposed to water, but it gets surrounded by non-
polar protein when the molecules bind together. If you are looking at these two molecules
under a microscope, what will you see if the molecules are separated? Bound? (Hint: the
proteins are both much too small to see under the microscope, but you can see the emitted
photons.)

Explanation / Answer

we will see fluoroscence according to the wavelength of light emmitted

for wave length

energy emitted = hc/ lambda = energy absorbed by photom

or, 2.3 *1.6*10^-19 = 6.6*10^-34 * 3*10^8 / lambda

so, lambda = wavelength of the emitted photons = 5.38 * 10^-7 m

so we will see green color

and this indicates that the complex is broken

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