Please answer the following multiple-part question regarding electrophoresis of
ID: 142503 • Letter: P
Question
Please answer the following multiple-part question regarding electrophoresis of nucleic acids:
a) You need to separate ssDNA molecules that are different from each other by one or a few nucleotides in size. You should use________ gel.
A. 1-2% native agarose
B. 2-3 denaturing agarose
C. 3-4% native polyacrylamide
D. 5-6% denaturing polyacrylamide
b) The electrolyte used in electrophoresis contains _______, the stains used in visualizing nucleic acids include _______ and the denaturants used in denaturing gel electrophoresis generally include _______.
A. NaCl/ methylene blue/ Clorox
B. Tris acetate/ ethidium bromide/ urea or formaldehyde
C. Tris borate/ acrydine orange/ sodium hydroxide or ammonia
Explanation / Answer
Answer a: 2-3 denaturation agarose.
Answer b: B. Tris acetate/ ethidium bromide/ urea or formaldehyde