In the space provided, indicate whether a light microscope [LM] or transmission
ID: 143835 • Letter: I
Question
In the space provided, indicate whether a light microscope [LM] or transmission electron microscope [TEM] would be best for visualizing each structure or event listed below (COULD SOMEONE PLEASE HELP ME WITH THE FOLLOWING TABLE ANSWERING EACH STRUCTURE OR EVENT)
Structure or event LM or TM Why? Motion of chloroplasts in plant cells Viral Particles Motion of Bacterial cells in animal cells Mitochondria in plant cell Nuclear pore Rough endoplasmic reticulum Cell viability (is the cell dead or alive?)Explanation / Answer
1)structure or event can be best resolve d with help of a light microscope LM
light microscope - It can resolve details which are even 0.2 micrometre apart.The average size of a cell is 10 to 20 micrometre dianetdi which is much less than the size of smallest particle that can be seen with naked eyes.The ultimate wavelength of resolution is set by visible light which ranges from 0.4 to 0.7 micrometre.
2)motion of chloroplast in plant cells TEM
Transmission electron microscope it requires an electron beam for resolution they can resove image upto 100nanometre small .The smaller de Broglie wavelength help in resolution.
3)viral particles
TEM
viruses are of different size so best way to visualize them is through electron microscope as they are of various range of size electron microscope can observe upto a wide range.
4)motion of bacterial cells in animal cells
TEM
it can resolve minute details so the best microscope to resolve them is TEM .Tem is resolves minute details.
5) mitochondria in plant cell
TEM
it can be best viwed by transmission electron microscope they are vaguely visible in loght microscope for details tem is required.
6)nuclear pore
TEm
tHey are best viewed in electron microscope . Because nucleus pore is minute and with details so it can be best described with tem
7)rough endoplasmic reticulum
Tem
is ideal for endoplasmic reticulum
TEM resolve s to high power so it resolves minute details
8) cell viability
TeM
it is best way light microscope reresolves tto a very low magnification compared to transmission electron microscope.