Can someone please check and help me with these? Especially II and III? I. Below
ID: 146408 • Letter: C
Question
Can someone please check and help me with these? Especially II and III?
I. Below is a DNA sequence that is a template strand and contains an Open reading frame for a gene:
3’ TAA ATA CGT GAG GCA CTT CAA AGA CCG CAC ACT_ AAT TT 5’
5’ AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3’
a. Transcribe the DNA template into a strand of mRNA by typing the mRNA sequence below the template strand. Label the 5’ and 3’ end of the mRNA 5’ AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA _ UUA AA 3’
b. Paste the mRNA below and do all 3 reading frames. Highlight all start codons green in all the reading frames. Highlight red the stop codons which are in frame with the start codons in all the 3 reading frames if they are present.
1) 5’ AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA UUA AA 3’
2) 5’ A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3’
3) 5’ AU UUA UGC ACU CCG UGA AGU UUC UGG CGU GUG AUU AAA 3’
c) In which reading frame is the ORF found? The second one. Since it has AUG.
d) Paste the mRNA showing the most likely ORF below and translate the codons. Label the 5’ and 3’ end of the mRNA and the N- and C-termini of the peptide.
5’ A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3’
MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP
N Terminus C Terminus
II. 3 pts. The base calling program made a mistake that you didn’t catch and deleted the bolded G in the original template strand above. Paste the template DNA below and delete the G. Transcribe (show the codons) and translate the resulting sequence. Label the 5’ and 3’ ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.
5’ AUUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A ‘3
MET HIS SER VAL LYS PHE LEU ARG CYS ASP STOP
III. 3 pts. The base calling program made a mistake that you didn’t catch and added an extra G after the bolded G in the original template strand above. Paste the template DNA below and insert the extra G. Transcribe (show the codons) and translate the resulting sequence. Label the 5’ and 3’ ends of the DNA and mRNA and the N and C-termini of the peptide. Comment on the resulting peptide sequence.
5’ AUUU AUG CAC UCC GUG AAG UUU CUG GGG UGU GAU UAA A ‘3
MET HIS SER VAL LYS PHE LEU GLY CYS ASP STOP
Explanation / Answer
I) B) Your answer is correct.
1) 5’ AUU UAU GCA CUC CGU GAA GUU UCU GGC GUG UGA UUA AA 3’
2) 5’ A UUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A 3’
Start Stop
3) 5’ AU UUA UGC ACU CCG UGA AGU UUC UGG CGU GUG AUU AAA 3’
II) What is the bolded G in your question? You did not show any G in bold. If any G is deleted in the middle of the ORF then the ORF will be changed. Suppose, if we consider G in the GUG is deleted the ORF will be changed.
5’ AUUU AUG CAC UCC GUG AAG UUU CUG GCG UGU GAU UAA A ‘3
5’ AUUU AUG CAC UCC GUA AGU UUC UGG CGU GUG AUU AAA ‘3
MET HIS SER VAL VAL SER PHE TRP ARG VAL ILE LYS
III) What is the bolded G in your question? You did not show any G in bold. If any G is added in the middle of the ORF then the ORF will be changed. Suppose, if we consider G after the GUG is added then the ORF will be changed.
5’ AUUU AUG CAC UCC GUG AAG UUU CUG GGG UGU GAU UAA A ‘3
5’ AUUU AUG CAC UCC GUG GAA GUU UCU GGG GUG UGA UUA A A ‘3
MET HIS SER VAL GLU VAL SER GLY VAL STOP