Chapter 8 ASSIGNMENT Evolution 1. The amino acid sequence for the globins (alpha
ID: 148635 • Letter: C
Question
Chapter 8 ASSIGNMENT Evolution 1. The amino acid sequence for the globins (alpha, beta and delta) are aligned below. The red letters in the beta and delta sequences show the locations of replacement. The yellow highlights show the replacements between the alpha and delta globins. Don't count the gaps. Solve for ?272 what % divergence is observed between beta and delta globin proteins? 13147-8.8% What % divergence is observed between alpha and delta globin proteins? ??? 140-m% 50 51 100 150 The nucleotide sequences for the same genes are aligned below. The red (underlined) letters represent non-synonymous replacements and the blue (italicized) letters are silent synonymous substitutions. Differences highlighted in the alphaglobin lines show alpha-delta divergence Differences highlighted in the hetaglobin lines show beta-delta divergence 51 NV.LSPpKT,! V K A a w G SDSHUNALPHAGL.OIN ATG GTG CDSHUMALTAGAPAN ATG GTG CAC CTG ACT CCT GAG GAG AAG TCT GCC GTT ACT GCC CTG TGG GGC SRSHUNPENTAGI OIN ATG GTG CAT CTG ACT CCT GAG GAG AAG ACT GCT GTC AAT GCC CTG TGG GGC CTG CT CCT GCC GAC AAG ACC AAC GTC AAg GCC gge TCG GGT HUNDELTAGLOBIN SDSHWMALPHAGLOIN AAG GTC GGC GCG CAC GCT GGC GAG TAT GGT GG GAG GCC CTG GAG AGGTG CpsMMMR TAGIONAM AAG GTG AAC GTG GAT GAA GTT GGT GGT GAG GCC CTG GGC AGG CTG SPAMMMPELTAGLORER AM GTG . . . . . . AAC GTG GAT GCA Grr GGT GGT GAG GCC CTG GGC AGATA GAC CDSHUMBETACLOBIN CTG GTG GTC TAC CCT TGG ACC CAG AGG CTC TTT GAG TCC TTT GGG GAT DSHUNDELTAGLOBIN CTG GTG GTC TAC CCT TGG ACC CAG AGG TC TTT GAG TCC TTT GGG GATExplanation / Answer
For amino acid sequence :
1.No.of replacements between alpha and delta protein =76
% divergence between alpha and delta = 76/140 = 54.2%
For Nucleotide sequence :
-No.of replacement positions between alpha and delta coding sequence=118
% divergence in relacement position for alpha and delta coding sequence = 118/334= 35.3%
-No.of silent positions between alpha and delta coding sequence = 44
%divergence in silent position for alpha and delta coding sequence = 44/113 = 38.9%
-Ka/Ks ratio for alpha globin and delta globin comparison = 35.3%/38.9% = 91%
-1 UEP = 10.4 million years
How many millions years ago did the alpha and delta globins begin to diverge = 35.3*10.4 = 367.12 MYA
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