Please explain step wise how to convert and what to do to get 20,000 U/ml to .04
ID: 149256 • Letter: P
Question
Please explain step wise how to convert and what to do to get 20,000 U/ml to .04 Units enzyme/ugDay 6 Obtain and Dissolve DNA Pellet Spin in centrifuge for 20 minutes at 4° C and 13100 K (as fast e Wash pellet with cold 0.75 EtOH. Spin 20 min at D should have a reasonably sized, whitish pellet (going t this stage, you should have approximately 50-70 m Test Digest Enzy T.Be careful, pellet may be slippery, so don't leave in : (if pellet's dissolved) 20,000 U/m Amt: 0.15m t2Use: .04 Units enzyme/ug DNA of Xbal let a. b. .0833 Units/ug Hind3 c. .125 Units/ug of Dral d. .125 Units/ug of Haell ta l amount of enzymes should be less tha oth of the total volume (enzymes+water tsmart buffer (NEB) he enzymes are purchased as 20,000 Uni Use Water if necessary (depending on co Cutsmart buffer should account for 1/10
Explanation / Answer
20,000 units/ml means 20,000 units in 1 ml of the enzyme.
0.04 units/ug DNA of Xbal means that for 1 ug of DNA, 0.04 units of the enzyme have to be used.
Now, we just need to calculate how many ml of the enzyme has 0.04 units.
1 ml --------------- contains---------------- 20,000 units
1/20,000------------------------------ 1 unit
(1/20,000)*0.04 ------------------------------ 0.04 units.
that means, for every ug of DNA, 2 x 10-6 ml of the enzyme has to be added. In other words, 2 ul of the enzyme per ug of the DNA.