Relevánt results Kin notawm ina dinuum . A culture contains 2.45 x 10 cells per
ID: 150165 • Letter: R
Question
Relevánt results Kin notawm ina dinuum . A culture contains 2.45 x 10 cells per milliter. A 1 ml volume of the culture was added to s naga sterile water in a bottle labeled "Bottle 1". A1 ml volume of "Bottle 1" was then added to 2" that contained 99 ml of sterile water. Finally,1mL of "Bottle 2" was added to water labeled "Bottle 3" This space left blank for you to take notes or make drawings as you feel necessa rn h00 2 a. Please indicate the total dilution factor for each bottle compared to the original Bottle Total Dilution Factor 2 If 1 mL and 0.1 mL were removed from each bottle and pour plated, how many would it be reasonable to predict to develop on/in each plate after the plates a incubated? b. Number of Colonies Bottle Volume Plated 0.1 mL 1 mL 0.1 mL 1 mL 0.1 mL 1 mL The culture rantains E. coli, which is a facultative anaerobe. Would the sameExplanation / Answer
13) A) Bottle Total dilution factor
1 102
2 104
3 105
B) Bottle Volume plated Number of colonies
1 0.1 ml 2.45 X 106
1 ml 2.45 X 107
2 0.1 ml 2.45 X 104
1 ml 2.45 X 105
3 0.1 ml 2.45 X 103
1 ml 2.45 X 104
14) A) 0.1 ml of bottle 1 contains 2.1 X 104 colonies.
Therefore, 1 ml of bottle 1 contains 2.1 X 105 colonies.
So, The original culture contains 2.1 X 105 X 100 = 2.1 X 107 CFU/ml