Total Mark I EXPERIMENT #2 30 Measuring Protein in Solution: The Bradford Assay

Question

Total Mark I EXPERIMENT #2 30 Measuring Protein in Solution: The Bradford Assay Lab Section Date Name: Include these sheets at the end of your Experiment #2 lab report. Circle the correct answer from choices shown in bold or fill in the blank 1. Proteins are polymers made up of amino acids linked together by pantide 2. When a bond forms between two amino acids, a alysosidic lester bonds. These bonds are is lost condensation Ihydrobais reaction. The free amino group at the end of amino acids commonly found in proteins what through a a polypeptide is referred to as the 3. There are 112 120 standard 4. The linear sequence of aminol 13distinguishes each amino acid is its g-carbonls acids forming a protein called the primary tertiary L quaternary structure. a-helices and B-pleated sheets are characteristic of primany Formation of a-helices and B-pleated sheets rely on g bonds which are weak I strong but there are a lot of them to provide structural 5. The overall 3-D shape of one polypeptide is called the pdary Lscondany tertiary / quaternary structure. This type of structure r R-groups Iove &Erisa) An example of a R-group interaction is a disulfide bridge Ioue Falae) To create a disulfide bridge, the side chains between both gytaaine svsteine relies on interactions between &. In the figure of human hemoglobin, each polypeptide (or "subunit) of this protein ia represented by a dfferent colour. The interaction between all subunits to form a complete protein is referred to as p structure. This protein is because there aresubunits (give number in the ban Figure 1: Human

Explanation / Answer

1. Peptide Covalent

2. Water Hydrolysis N-Terminus

3. 20 Side chain (R group)

4. Primary Secondary Hydrogen weak alpha -helix(the arrow structure in the figure)

5. Tertiary True True Cysteine Ionic

6. Quatenary Tetramer 4

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