CHM5600-Winter 2013 Second Midterm Exam Mark your answers on the cover sheet. On

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CHM5600-Winter 2013 Second Midterm Exam Mark your answers on the cover sheet. Only answers on the signed cover sheet will be scored. S1: Draw a dG-dC base pair. Show the entire nucleotide. Indicate where the S2: Draw phosphatidylethanolamine. Use 16:0 and 18:3 412.15 as the fatty S3: In the space provided, calculate Ki the reaction shown in Figure 1 major and minor grooves would be located. (20 pts) acid chains. (10 pts) Show your work for full credit. Please note that multiple choice questions 1-4 also relate to this plot. (20 pts) S4: Compare and contrast. Biology has several forms in which it can store energy. Consider three forms of sugar storage: free glucose, starch and glycogen. Compare and contrast the structures of these three energy storage molecules? In relation to these structures, discuss the pros and cons for the organism/cell were it to store its glucose reserves in each of these three chemical forms. How does the structure of the molecule dictate these favorable and unfavorable aspects of storing glucose reserves in this way? (30 pts) Figure 1. Inhibitor concentrations 0,9 and 20 nM for rns A, C, and E respectively. Rates are in uM/sec and [S) is in units of uM. Least-squares fitting of the lines are provided Inhibition Data y 25x 0.3125 y#25x + 05417 y2.5x+0.125 1.8 1/rate-A 8 Linear (/rate A) Linear (1/rate-C) Linear (1irate-E) 14S] p. 2 of 8

Explanation / Answer

1. The Vmax for no inhibitor, will be for reaction A i.e. with 0 concentration of the inhibitor.

1/Vmax= the intercept on Y-axis= 0.125

So, Vmax= 8 um/sec (E.)

2. E. Irreversible Uncompetitive inhibition

These inhibitions are considered as irreversible because the inhibitor binds to the Enzyme substrate complex and inhibits the rection from proceeding in the reverse direction.

3. In this type of inhibition, the inhibition site is generally accessible to the inhibitor after the enzyme has been bound to the substrate. So, a high substrate concentration (B.) is most suitable for this kind of inhibition.

4.For an uncompetitive inhibition, both the Vmax and Km of the reaction are reduced upon addition of the inhibitor.

So, Plot A represents the type of curve that will be the best illustration of the Michaelis-Menten Curve for this type of inhibition.

All the Best :)

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