Consider cargo proteins that are destined for secretion to the extracellular spa

Question

Consider cargo proteins that are destined for secretion to the extracellular space. For each of the following pairs of mutations or conditions predict where the cargo will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple places, extracellular space) for: (i) & (ii) Each individual condition, & (iii) The combination of both. Normal Sar1 is mutated so that it hydrolyzes GTP immediately after binding GTP. The signal recognition particle is mutated to be unable to bind to the SRP receptor. Both A mitochondrial signal sequence is added to the C-terminus of the cargo protein. v-SNAREs on COPII coated vesicles are mutated to be unable to bind t-SNAREs Both COPII coat proteins are mutated so they don't bind to the ER membrane. COPI coat proteins are mutated so they don't bind to the Golgi membrane. Both Normal Sar1 is mutated so it binds tightly to GDP and cannot exchange it for GTP. (This leads to Sar1"-GDP being the only form of the protein in the cell.) A domain is added to the cargo protein. Both

Explanation / Answer

5a)i)binding gtp and hydrolizing it ends up in cytoplasm as it is the fluid where GTP is found

ii)It will be present is transport vesicles as the signal receptor is mutated in vesicles before transportation.

iii)the combination of both is too big so will be present in the extracellular space.

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