An oligomeric protein has a molecular weight of 443000. A 610 mg sample was trea
ID: 163705 • Letter: A
Question
An oligomeric protein has a molecular weight of 443000. A 610 mg sample was treated with an excess of 1-fluoro-2.4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCI. the product of the complete hydrolysis contained 1.3 mg of the compound shown below, and no other 2.4-dinitrophenyl derivatives of the a-amino groups of other amino acids could be found, how many polypeptide chains are in this protein? (Enter your answer as a whole number).Explanation / Answer
Molecular weight of given protein is 443000
Complete hydrolysis = 1.3/443000 = 2.934
Treated sample = 610/443000 = 0.001
Remaining polypeptide chains in protein = 2.934/0/001 = 3.408
Therefore, we got 3 polypeptide chains after complete hydrolysis.