Please answer part three in a long paragraph PLEASE! Project 1 Part of the CFTR

Question

Please answer part three in a long paragraph PLEASE! Project 1 Part of the CFTR gene sequence NOTE-make sure you print or view this page in color! 5' GATGcc AATGGAGTGATCTcGATCAAGccGGAGCTTcTCTTAACTTcGATTTCGAC GTGAAGAAC TTACTTTGATTGccACATTGGcccAATTGAAGGGTTATAAT CTcGCTGGGCATTTTGcTcGAATTGCAGTTTAGTTAATTTcGCTTCTTTc TCAGTAAATAATAAcTGTGCATcGAAGGcCAACTGGAGATGGAAGTGGAA. GTCATTAATATcGATATACAAGAAGTACAATACAACAGTAAGTTATTAcc TA ATA. TTTTTTTTTTAAATATTTAGG TTCAAATAMATTAGcCATAGTTATATATTTTTTATGTTTTCATTTACATA AAACATTAACTGTTATTTTTTTAAATTTAALINGGACTAGcTTTTTCAATTG TACAGCACAccCT GTGGCTGcGAGTANcGGTTCTCGGGAGTOGTTATTTT GONAATGITTATGTACAAATATTGAAT AAAAAA ATAA COAGCCTCATOGATOTccari GCAAGGAGACOTGCATTOGcCACTRTCAcaccACCAAGAAGCAacTGcaA. SAMSUNG Concepts in Biology AATCTCT Deleted bases in B changed to A in some patients B changed to T in some patients changed to C in some patients

Explanation / Answer

Hi, we are considering mutation 4 and the primers are as follows

Forward Primer acts on 3' to 5' orientation of DNA - antisense strand. Length - 19bp

Reverse Primer acts on 5' to 3' orientation of DNA - sense strand. Length - 19bp

The product size after amplication is 251 bp length.

Note: Count(G + C)/Count(A + T + G + C) * 100.

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