In the DNA \"Haystacks\" below you are given a normal DNA strand and it\'s alter
ID: 166968 • Letter: I
Question
In the DNA "Haystacks" below you are given a normal DNA strand and it's altered but similar DNA strand. Your first job is to analyze each pair and identify and name the type of genetic "alteration". Next, use the mRNA codona Amino Acid chart to decode both strands and identify exactly how the "alteration" changed or confused "genetically coded message". Normal "Sense" Strand Tilde ATGAAAAACAAGGTACACATCTAG mRNA Strand Tilde _______ Polypeptide Tilde _______ Altered "Sense" Strand Tilde ATGAAAAACAACGTACACATCTAG mRNA Strand Tilde _______ Polypeptide Tilde _______ Circle the "altered" section and identify the kind of alteration: _______ Normal "Sense" Strand Tilde ATGAAAAACAATTGCACGTAG mRNA Strand Tilde _______ Polypeptide Tilde _______ Altered "Sense" Strand Tilde ATGAAAAACAATTGCACGTG mRNA Strand Tilde _______ Polypeptide Tilde _______ Circle the "altered" section and identify the kind of alteration: _______Explanation / Answer
Ans. A. #1 Normal “Sense strand”: ATGAAAAACAAGGTACACATCTAG
The sequence of mRNA is exactly the same as sense DNA strand, except T in DNA is replaced by U in mRNA.
So, mRNA strand = AUGAAAAACAAGGUACACAUCUAG
Polypeptide: Met- Lys- Asn- Lys- Val- His- Ile- STOP
A. #2. Altered: Normal “Sense strand”: ATGAAAAACAACGTACACATCTAG
mRNA strand = AUGAAAAACAACGUACACAUCUAG
Polypeptide: Met- Lys- Asn- Asn- Val- His- Ile- STOP
Point mutation, Missense mutation (A mutation that changes type of amino acid being coded).
B. #1. Normal “Sense strand”: ATGAAAAACAATTGCACGTAG
mRNA strand = AUGAAAAACAAUUGCACGUAG
Polypeptide: Met- Lys- Asn- Asn- Cys- Thr- STOP
B. #2. Altered “Sense strand”: ATGAAAAACAATTGCACGT //A// G Note: - //A// deleted
mRNA strand = AUGAAAAACAAUUGCACG UG
Polypeptide: Met- Lys- Asn- Asn- Cys- Thr-
Deletion mutation, frameshift Mutation (changes reading frame due to insertion or deletion of one or more nucleotides, not 3 or its multiple).