Can you please answer the data analysis Thank you very much Effects of salinity
ID: 168892 • Letter: C
Question
Can you please answer the data analysisThank you very much Effects of salinity on Molly Metabolic Rates Modified from: Beck, C. L Blumer and T. Brown. 2003. Effects ofsalinity on metabolic rate in black studies for laboratory 24 Donnell, Editor). mollies. Pages workshop/conference of the Association for B Laboratory Education. Proceedings of the 24th nt or actively regulate internal water and Aquatic organisms either conform to their regulators living in freshwater, their body fluids are solute levels to maintain their water balance. For the to absorb water and lose solutes. To maintain their water balance, freshwater regulators produce large amounts of dilute urine and actively retain and take up solutes. Among both freshwater and saltwater re the active transport of solutes against an osmotic gradient is energetically expensive. result, as the osmotic stress on an individual increases, we would expect to see an increase in metabolic rate. However, under extreme osmotic stress, individuals cannot maintain their water balance, and physiological systems shut down, leading to a decrease in metabolic rate. Therefore, forfreshwater organisms, we would expect a increase in metabolic rate from low to moderate salinities, and then a decrease in metabolic rate at high salinities. contrast, we would expect to see those found in their natural depressed metabolic rates salinities lower and freshwater Although most organisms have physiological systems adapted to either saltwater habitats, some species living in tidal areas (e.g. the wild relatives of black mollies in their natural environment) experience daily fluctuations in salinity (usually between 1 and 10 parts thousand or ppt Therefore, they must be able to adjust to changes their environment and we would expect them to have a wider range of salinity tolerance. OBJECTIVES In this experiment, you will Evaluate metabolic rate of a fish by measuring direct oxygen consumption Use class data to evaluate the metabolic rate pattern of an intertidal fish in a range ofsalinities PROCEDURE 1. The Dissolved oxygen Probe should have already been filled and be sitting in a smalf of distilled water. Tap on the meter screen (to right) and set duration 15 ith a rate of 12 samples per minute. Select OK. Tap the arrow key to start data collection so the probe can warm up. 2 the probe warming up, fill a 125 mLErlenmeyer flask (up to the line with water from the beaker of appropriate test salinity (start with 1ppt fish tank water, then do 10ppt, and do 20ppt and 30 ppt if you have time) Add the magnetic stir bar and get the spinning low so the but the will be reasonably stirred. Hopefully we can agree on a rate and stick to that rate across groups. Once set, do NOT change stirrate between treatments. Add the fish and insert the oxygen probe. Seal the system with parafilm making sure that no air at the nask may and with the parafilm. Restart the data collection and allow the fish to adjust for 5 minutes. The fish may be active pproximate initial ally, which would increase the metabolic rate. After 5 minutes note the a oxygen concentration in Table 1 collect data for 15 minutes. When the 15 minutes are up, note 'final concentration in Table 1 and then select the most linear 5 minutes from the last 10 minutes of n for that time period. lyze to get the slope of the linear data collection and choose ana Record the slope in Table 1
Explanation / Answer
1. we can able to estimate a metabolic rate using oxygen consumption in water. ther should not be no air at the top of the fish flask as tthe oxygen level in the air may disturb the experiment causing fluctuations in the metalabolic rate.
2. there is a slight effect of the salinity in estimated MR. as the experience daily fluctuations of sality should vary between 1 to 10 ppt.
3. time calculation for noting down the oxygen level.