Part B To test the hypothesis that the three conserved regions function as regul

Question

Part B

To test the hypothesis that the three conserved regions function as regulatory DNA, you make several transgenic constructs using a GFP reporter gene and you evaluate their expression in developing heart and lung tissue.

Construct 1 is the wild-type construct. Notice that this constuct has normal expression in both tissues.

For Constructs 2-8, you introduced point mutations into each of the three conserved regions (indicated by ***). Expression results for each of these constructs are indicated in the chart.

Drag the best answer from the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all.

necessary but not sufficient

necessary and sufficient

no region

Region 1

Region 2

Region 3

1. Region 1 is A: for gene expression in the heart because expression is absent when this region is mutated.
Which other region, if any, is also required for gene expression in the heart? A:

2. Region 2 is A: for gene expression in the lung because expression is absent when this region is mutated.
Which other region, if any, is also required for gene expression in the lungs? A:

3. Region 3 is A: for gene expression in the heart because expression is absent when this region is mutated.
Which other region, if any, is also required for gene expression in the heart? A:

necessary but not sufficient

necessary and sufficient

no region

Region 1

Region 2

Region 3

1. Region 1 is A: for gene expression in the heart because expression is absent when this region is mutated.
Which other region, if any, is also required for gene expression in the heart? A:

2. Region 2 is A: for gene expression in the lung because expression is absent when this region is mutated.
Which other region, if any, is also required for gene expression in the lungs? A:

3. Region 3 is A: for gene expression in the heart because expression is absent when this region is mutated.
Which other region, if any, is also required for gene expression in the heart? A:

Construct 1 Construct 2 Construct 3 Construct 4 Construct 5 Construct 6 Construct 7 Construct 8 GFP GFP in GFP in heart lung tissue tissue present present absent present present absent absent present absent absent absent absent absent present absent absent

Explanation / Answer

Answer:

1 Region 1 is necessary but not sufficient for for gene expression in the heart because expression is absent when this region is mutated.

Which other gene region, if any, is also required for gene expression in the heart - Region 3

2. Region 2 is necessary and sufficient for gene expression in the lung because expression is absent when this region is mutated.

Which other region, if any, is also required for gene expression in the lungs - no region

3. Region 3 is necessary but not suffcient for gene expression in the heart because expression is absent when this region is mutated.

Which other region, if any, is also required for gene expression in the heart - Region 1

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