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Please Help. Biology Question. You find a new gene involved in metabolizing plas

ID: 174071 • Letter: P

Question

Please Help. Biology Question.

You find a new gene involved in metabolizing plastics. You decide to search for other potential plastic metabolizing bacteria by using primers that amplify that sequence. When you perform PCR on your different sample sets from local landfills, you find that every sample is positive for the plastic degrading gene of interest. Unfortunately you also realize that you forgot a negative control, and are concerned you contaminated your samples. What would be an effective negative control? No Sample No Buffer No dNTPs No polymerase

Explanation / Answer

No polymerase

Explanation:

3 most common negative controls include

1.No DNA polymerase

2. No template DNA

3. No reverse transcriptase