Colony when grown on medium containing the indicator X-gal? Briefly explain why
ID: 176417 • Letter: C
Question
Colony when grown on medium containing the indicator X-gal? Briefly explain why the bacteria would form a colony of the color you chose. Suppose you had a cell with a mutation in the gene that encodes the Iron Response Factor (IRF) protein, which resulted in this protein being able to bind to its binding site, the Iron Response Element (IRE), under both low and high intracellular iron conditions. What effect would this mutation would have on the cell's production of the iron transport protein transferring? Briefly explain why you expect the effect you predict.Explanation / Answer
Iron overload disease due to the mutation of the FERROPORTIN has a dominant inheritance and a variable clinical phenotype such as some people show early Kupffer cell iron loading and low transferrin saturation, while some show Hepatocyte iron Loading and high transferrin saturation. The mutant protein falls into two main class- One that are not able to localize on the cell surface and hence unable to export iron, while others are those that localize to the cell surface but are unable to respond to the anti-microbial peptide hepcidin.
Patients with mutant Ferroportin proteins which do not localize to the cell surface show typical Ferroportin disease with low transferrin saturation and early Kupffer cell iron loading, while patients with mutant proteins unable to respond to hepcidin show high transferrin saturation and early hepatocyte iron loading.
People with abnormal iron regulatory genes do not reduce their intake of iron in response to the increased level of iron in the body. This leads to increased store of iron in the body and therefre, the iron which was initially stored as Ferritin, is now stored as Hemosiderin which is toxic to tissues, as it induces OXIDATIVE STRESS. Iron is a Pro-oxidant.