Can someone explain to me what is happening in this picture? I know that a sampl
ID: 176710 • Letter: C
Question
Can someone explain to me what is happening in this picture? I know that a sample of tissue is taken and the sample contains A and B alleles. The tumor shows the B allele as well. What can be explained from the electrophoresis results. I don't know how to word it correctly cton Figure 17.6 Polymorphic enzymes encoded by the X chromosome reveal the clonal origin of tumors. Each individual cel in a lomalo exprossos only ono form of a polymorphic XHinked gene because of X -chromosome inactivation, A patch of us for tissue will usually contain both types of cells, lf single cells are grown r the into a clono, they adhibit ono or the other enzyme form. A tumor also exhibits only one form, demonstrating that it arose from a single cell. The two alleic forms of the gene's protoin product are distinguished by oloctrophorosis. XAXB Female Sample of tissue Clone 1 of Clone 2 of cells Electrophoresis to separate allelic forms of enzymeExplanation / Answer
Polymorphic enzymes are two or more varient of an enzyme in a population encoded by a single gene locus. They vary slightly in their amino acid sequence but their catalytic property is similar. Due to cahnge in aminoacid sequence they migrate differently under electrophoresis.
Here X chromosome encoded two varient of an enzyme. Each cell has single variety of enzyme therefore these cells can be differentiated in a tissue.
In first case, tissue sample- means in tissue both type of cellsand hence both varient of enzyme protein present and when saprated by electrophoresis, two bands can be seen.
In second and third case, only one varient of enzyme from single type of cell undergone electrophoresis therefore a single band can be obsereved in both the cases.
As mentioned that tumor arises from a single type of cell, that means tumor is having only one varient of the enzyme so when enzyme protein from tumor is seperated via electrophorosis,it will give a single band.This band may be similar to second case ( clone 1 normal cell - one varient of enzyme) or third case (clone 2 normal cell-other varient of enzyme). Here in figure only one band similar to clone 2 normal cell is shown.