Please explain each of the following: -large T Antigen -original p53 data sugges
ID: 177697 • Letter: P
Question
Please explain each of the following:
-large T Antigen
-original p53 data suggested it was an oncogene why?
-p53 is a tumor suppressor gene : discovery
-Hetrozygous mutation of P53 also has phenotype . why? (unlike Rb)
-Tetramer structure
-Domain structure of p53.
-Common mutations leading to phenotype.
-Levels of p53 in nucleus; steady state level regulation.
-p-53 Activating signals
-downstream effects of p53
-p21 and p53
-regulation of p53 by MDM2
-regulation by ARF
-Kinases that control p53
-What is ARF , REGULATED BY?
-E2F regulated by?
-Consequences of p563 regulation in cancer?
-P53 and its effects on G1; S and G2/M phase.
APOPTOSIS:
-Cellular/organelle changes in apoptosis
-Apoptotic pathways: intrinsic and extrinsic
-Key proteins in apoptosis
-Bcl2 family
-Location and function of each,
-Cytochrome C
-Apoptosome formation
-Death receptor and ligands
-Cancer and apoptosis
Explanation / Answer
large T Antigen:
original p53 data suggested it was an oncogene why?
p53 is a tumor suppressor gene : discovery
Domain structure of p53:
The p53 protein is a phosphoprotein made of 393 amino acids. It consists of domains:
-regulation of p53 by MDM2: