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Please answer the following genetics / agronomy questions: 24. In whole transcri

ID: 177892 • Letter: P

Question

Please answer the following genetics / agronomy questions:
24. In whole transcriptome sequencing experiments we should not exprect to see expression of all genes on the genome as expression of genes are spatially and temporally and environmentally regulated and it may be that some genes are not expressed during our experiment
a) True b) False
25. In whole transcriptome sequencing experiments we observe expression of all genes on the genome because even gene expression is spatially and temporally and environmentally regulated, RNA-seq is able to sequence those mRNAs
a) True b) False
Please answer the following genetics / agronomy questions:
24. In whole transcriptome sequencing experiments we should not exprect to see expression of all genes on the genome as expression of genes are spatially and temporally and environmentally regulated and it may be that some genes are not expressed during our experiment
a) True b) False
25. In whole transcriptome sequencing experiments we observe expression of all genes on the genome because even gene expression is spatially and temporally and environmentally regulated, RNA-seq is able to sequence those mRNAs
a) True b) False
Please answer the following genetics / agronomy questions:
24. In whole transcriptome sequencing experiments we should not exprect to see expression of all genes on the genome as expression of genes are spatially and temporally and environmentally regulated and it may be that some genes are not expressed during our experiment
a) True b) False
25. In whole transcriptome sequencing experiments we observe expression of all genes on the genome because even gene expression is spatially and temporally and environmentally regulated, RNA-seq is able to sequence those mRNAs
a) True b) False
Please answer the following genetics / agronomy questions:
24. In whole transcriptome sequencing experiments we should not exprect to see expression of all genes on the genome as expression of genes are spatially and temporally and environmentally regulated and it may be that some genes are not expressed during our experiment
a) True b) False
25. In whole transcriptome sequencing experiments we observe expression of all genes on the genome because even gene expression is spatially and temporally and environmentally regulated, RNA-seq is able to sequence those mRNAs
a) True b) False
Please answer the following genetics / agronomy questions:
24. In whole transcriptome sequencing experiments we should not exprect to see expression of all genes on the genome as expression of genes are spatially and temporally and environmentally regulated and it may be that some genes are not expressed during our experiment
a) True b) False
25. In whole transcriptome sequencing experiments we observe expression of all genes on the genome because even gene expression is spatially and temporally and environmentally regulated, RNA-seq is able to sequence those mRNAs
a) True b) False

Explanation / Answer

24. True. Whole transcriptome sequencing experiments we should not expect to see expression of all genes on the genome as expression of genes spatially and temporally and environmentally regulated and it may be that some genes are not expressed during our experiment.

25. a) True. Gene expression is spatially and temporally and environmentally regulated. RNA-seq is able to sequence those mRNA.

b) RNA-seq is used to analyze the continually changing cellular transcriptomes. It depends on alternative splicing, post-transcriptional modification, gene fusion, mutations, and changes in gene expression.

c) RNA-seq also looks at different types of RNAs such as miRNA, tRNA, ribosomal profiling and also determines intron/exon boundaries.