Please answer the following genetics questions: 18. Which statement is correct?
ID: 177895 • Letter: P
Question
Please answer the following genetics questions:18. Which statement is correct?
a) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. b) Talen technology is considered as forward genetic approach because it creates a new variation. c) In forward genetic approach, we determine the function of gene by analyzing the phenotypic effects of altered DNA sequences. d) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on post-transcriptional regulation of gene expression.
19. Which statement is false?
a) In CRISPR-cas system the gRNA guide the cas protein to the exact target sequence and then cas cleavage site will cut the target DNA on both strands. b) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. c) In nature, the repeat-spacer segment of the CRISPR-cas defense system in bacteria, can be added to the cell memory every time cell is attacked by a new virus. This addition is through incorporation of viral sequence recognition site to the bacterial chromosome where other repeat-spacers are located. d) In a knock-out mouse the gene is expressed into mRNA but a mechanism prevents the process that translates mRNA into protein.
Please answer the following genetics questions:
18. Which statement is correct?
a) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. b) Talen technology is considered as forward genetic approach because it creates a new variation. c) In forward genetic approach, we determine the function of gene by analyzing the phenotypic effects of altered DNA sequences. d) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on post-transcriptional regulation of gene expression.
19. Which statement is false?
a) In CRISPR-cas system the gRNA guide the cas protein to the exact target sequence and then cas cleavage site will cut the target DNA on both strands. b) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. c) In nature, the repeat-spacer segment of the CRISPR-cas defense system in bacteria, can be added to the cell memory every time cell is attacked by a new virus. This addition is through incorporation of viral sequence recognition site to the bacterial chromosome where other repeat-spacers are located. d) In a knock-out mouse the gene is expressed into mRNA but a mechanism prevents the process that translates mRNA into protein.
18. Which statement is correct?
a) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. b) Talen technology is considered as forward genetic approach because it creates a new variation. c) In forward genetic approach, we determine the function of gene by analyzing the phenotypic effects of altered DNA sequences. d) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on post-transcriptional regulation of gene expression.
19. Which statement is false?
a) In CRISPR-cas system the gRNA guide the cas protein to the exact target sequence and then cas cleavage site will cut the target DNA on both strands. b) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. c) In nature, the repeat-spacer segment of the CRISPR-cas defense system in bacteria, can be added to the cell memory every time cell is attacked by a new virus. This addition is through incorporation of viral sequence recognition site to the bacterial chromosome where other repeat-spacers are located. d) In a knock-out mouse the gene is expressed into mRNA but a mechanism prevents the process that translates mRNA into protein.
18. Which statement is correct?
a) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. b) Talen technology is considered as forward genetic approach because it creates a new variation. c) In forward genetic approach, we determine the function of gene by analyzing the phenotypic effects of altered DNA sequences. d) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on post-transcriptional regulation of gene expression.
19. Which statement is false?
a) In CRISPR-cas system the gRNA guide the cas protein to the exact target sequence and then cas cleavage site will cut the target DNA on both strands. b) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. c) In nature, the repeat-spacer segment of the CRISPR-cas defense system in bacteria, can be added to the cell memory every time cell is attacked by a new virus. This addition is through incorporation of viral sequence recognition site to the bacterial chromosome where other repeat-spacers are located. d) In a knock-out mouse the gene is expressed into mRNA but a mechanism prevents the process that translates mRNA into protein.
Explanation / Answer
18. Which statement is correct?
a) T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression.-answer
Insertional mutagenesis is an option method for disturbing quality capacity and depends on the inclusion of remote DNA into the quality of intrigue. In Arabidopsis, this includes the utilization of either transposable components . The remote DNA not just upsets the declaration of the quality into which it is embedded additionally goes about as a marker for resulting recognizable proof of the transformation. Since Arabidopsis introns are little, and on the grounds that there is next to no intergenic material, the inclusion of a bit of T-DNA on the request of 5 to 25 kb long by and large delivers a sensational disturbance of quality capacity. On the off chance that a sufficiently expansive populace of T-DNA–transformed lines is accessible, one has a decent possibility of finding a plant conveying a T-DNA embed inside any quality of intrigue. Transformations that are homozygous deadly can be kept up in the populace as heterozygous plants.
The inclusion of a T-DNA component into an Arabidopsis chromosome can prompt to a wide range of results
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19. Which statement is false?
d) In a knock-out mouse the gene is expressed into mRNA but a mechanism prevents the process that translates mRNA into protein. -answer