Milk is used to unbind proteins that are embedded in a nitrocellulose O True OFa
ID: 180317 • Letter: M
Question
Milk is used to unbind proteins that are embedded in a nitrocellulose O True OFalse Gravity used to propelprotein trouth acrylamide apt IT APS is used as a catalyst to start the polymeration O True OFalse In SDS-PAGE, the tris-glycine-SDS buffer serves as the mobile True False I Mistakes in the lab book must be documented by a single strike-through. a date, and initials. OTrue OFalse 20 close toes shoes are required in the laboratory 210 Intense sound waves can be used to break down bacterial cell walls without damaging the protein of interest. O True OFalse 2% In the bacterial inclusion bodies. the concentration of excess protein is so high that the protein will denature and agglutinate. O True 2) Green fuorescent protein under visible ight Apo) 24 Nitrocellulose membranes must be activated with alcohol before the A membrane will bind protein. TrueExplanation / Answer
15 . True
Milk is a blocking agent. It looses protien. skimmed milk protein solution is a blocking solution.
16. False
The running of the proteins is not by gravity. It is because of thier negative charges of the gel.
17. True
Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide.
18. True
. The mobile buffer ions are Tris+, Gly-, and dodecylsulfate- (pH 8.3). thus tris glycine Sds buffer serves as a mobile phase.
19. false.
Errors crossed out with
mistakes crossed with a single line and explained.
20 true
In laboratories must wear closed-toe shoes, that cover the top of the foot that resists rapid penetration by spilled liquids or sharps.
21 False
It can rupture the some of the protien of interest.