Can someone please explain this to me? And why, whichever answer you pick, is th
ID: 183742 • Letter: C
Question
Can someone please explain this to me? And why, whichever answer you pick, is the right one!
You calculate the ratio of C-terminal radioactivity (radioactivity due to the C-terminal glutamine incorporated into the polypeptides) to the total radioactivity of the entire polypeptides (total radioactivity due to each of the five possible glutamine locations) over time, starting at the experiment with two minutes of growth in the presence of radioactive glutamine and ending with the experiment with 80 minutes of growth in the presence of radioactive glutamine. With increasing time of exposure of the cells to the radioactive glutamine, the ratio of C-terminal radioactivity to total radioactivity in the isolated proteins should: A Remain constant at a value of 0.2 (1/5 of radioactivity is due to the C-terminal glutamine residues) B) Increase to a final value of 0.20 (1/5 of radioactivity is due to the C-terminal glutamine residues) Decrease to a final value of 0.20 (1/5 of radioactivity is due to the C-terminal glutamine residues) Increase to a final value of 100% (all of the radioactivity will be due to the C-terminal glutamine residues) E Increase to a final value of 0.25 (1/4 of radioactivity at the C-terminal glutamine residue)Explanation / Answer
the ratio of C terminal radioactivity to the total radioactivity of entire polypeptide will increase with time exposure by 0.2. As with increase in time exposure number of the labeled polypeptide will be expected to increase.