IinB Ul ule Union epiderma Remember this from Lab 1.) In your drawing, clearly i
ID: 187827 • Letter: I
Question
IinB Ul ule Union epiderma Remember this from Lab 1.) In your drawing, clearly indicate the lo diameter of the cell used in determining this magnification. Show you Onior 30 e field of view/number of cells that fit 0.4/2 0.2mm Actual size 0.2mm = 200um Magnification of drawing measured size/actual size 30000um/200um 150X Q7. Answer the following questions about acetocarmine: - Is acetocarmine positively or negatively charged in solution? Positively charged Which organelle does it primarily stain? Chromosomes - Which component of this organelle is acetocarmine binding to, and why?Explanation / Answer
Acetocarmine is a basic dye that is primarily used to stain chromosomes during mitosis.
It is positively charged in the solution and is used to demonstrate the presence of negatively charged DNA in the chromosomes. 2% acetocarmine stain is widely used for staining meiotic chromosomes.
2% carmine preparation for 100 mL:
Glacial acetic acid = 45 mL
Distilled water = 55 mL
Carmine powder = 2 g