Please show work! Thanks! . A generalized transducing b c\'d\'e\' donor. The re
ID: 195664 • Letter: P
Question
Please show work! Thanks!
. A generalized transducing b c'd'e' donor. The re phage is used to transduce an a bede recipient strain of E, coli with an recipient culture is plated on various media indicated in the second column o with the results shown in the third column. (Note that "a" indicates a requirement for A nutrient, etc.) Experiment Compounds added umber to minimal medium Presence (+) or absence (-) of colonies CDE BDE BCE BCD ADE ACE ACD ABE ABD ABC 3 4 10 What genotype is being selected for in each experiment above? Which of the above markers can be cotransduced? What can you conclude about the linkage and order of the five genes?Explanation / Answer
a. In the experiment, if a compound is not added to the growth medium and the growth occurs, E. coli has received the genes for it by transduction. Hence, experiment 1 is selecting for a+b+ recombinants, experiment 2 for a+c+, 3- a+d+ , 4- a+e+, 5- b+c+, 6- b+d+, 7- b+e+, 8- c+d+, 9- c+e+, 10- d+e+.
b. Cotransduction is the process by which two genetic markers are simultaneously packed in a bacteriophage for transferring to new host bacterium. Hence from the experiments (1-10), only BDE, ACE and ACD showed colonies suggesting that a and c, b and d, and b and e can be cotransduced.
c. BDE culture must have received a+ and c+ , ACE must have received b+ and d+ and ACD must have received b+ and e+. b, d and e are closely related to each with b in the middle. a and c are closely related to each other, but far from b, d and e.