Academic Integrity: tutoring, explanations, and feedback — we don’t complete graded work or submit on a student’s behalf.

Can anyone please help me with the following question. Please I don\'t want the

ID: 200170 • Letter: C

Question

Can anyone please help me with the following question. Please I don't want the answer in scientific notation .

At 3:00 PM, you inoculate 1 x 103 cells in a closed flask on nutrient broth. The cells have a lag phase that lasts an hour. At 8:00 PM, the culture enters stationary phase. At that time, there are 6.5 x 107 cells in the flask. Calculate the number of generations (n), the number of generations/hour (k) and the doubling time or number of hours/generation (g).

n=?

k=?

g=?

I calculated n and it was 15.88 which showed it was correct while i calculated k which is 3.176 and g was 15.11 and both my answers for k and g were wrong. can anyone please help me.

Explanation / Answer

i think you considered the growth to be in 5 hours. do not consider 1 hour of lag phase.

try putting no. of generations/hr = 15.88/4 = 3.97

but the doubling time is still coming out to be 15.11 minutes. So, try to convert it into minutes to 15.6 (6 being minutes)