Here is the question and a hint. can anyone help to find an answer and provide a
ID: 200891 • Letter: H
Question
Here is the question and a hint. can anyone help to find an answer and provide an explanation?!!
HINT:
Time = 0 min. Time = 20 min 1. You are investigating a metabolic pathway in wildtype (WT) and mutant 3 (M) Starbucksocytes. Specifically, you S are interested in the conversion of Milk to Crappachinos. You take radiolabeled Milk, feed it to either WT (dark bars) or 1 (light bars) Starbucksocytes, and follow the products that are formed Your data is shown. What is the MOST LIKELY metabolic pathway(s) for conversion of Milk to Crappachinos? MOST LIKELY which enzyme(s) in the pathway is mutated in the M Starbucksocytes? (HINT: Not all pathways are linear!) Time = 10 min. Time = 30 min 0 9Explanation / Answer
C is the most likely metabolic pathway.
In the WT strain -
First, espresso and capuccino are produced in equal amounts. Then, 20mins after start of reaction latte and americano are produced from espresso and capuccino (everything is being produced in equal amounts). 30mins after start of reaction, huge surge in latte production is seen (better explained by C than D. If D was correct we would see surge in americano production. A and B do not explain the surge) crappachino is also produced from the latte.
Enzymes most likely to be mutated in M Starbucksocytes are:
the one that converts milk to espresso as in the M strain large amount of espresso can be seen at 20min.
enzyme that converts espresso to latte has probably also mutated as espresso is being retained in large quantity and concentration of latte is low at 30mins.