Meeps are unique in that they can eat silicone-based chemicals as well as carbon
ID: 201499 • Letter: M
Question
Meeps are unique in that they can eat silicone-based chemicals as well as carbon-based chemicals. From plain sand, they can create two different final products that enhance the little bobbles on the tips of their antennae. Wild type meep antenna bobbles have shiny, jewel-like facets. Meeps who cannot process sand properly have antennae that are either dull instead of shiny, round instead of faceted, or both. You collect a bunch of meeps that represent these phenotypic categories and establish three true-breeding lines of each phenotype (A-I).
Meep mutations A, B, and C: dull and nonfaceted
Meep mutations D, E, and F: dull, faceted antennae
Meep mutations G, H, and I: shiny, nonfaceted antennae
Wild type meeps: shiny, faceted antennae
You then perform crosses to determine how many genes are involved in sand processing. The data below contain the F1 antennae phenotypes of the hybrid offspring from crosses between true-breeding parent lines listed above.
1. How many complementation groups are there? _____
2. List the complementation groups below (which mutant lines are in each group):
3. What does a complementation group represent?
You discover that meeps turn the sand into a number of intermediate chemicals. L and M are both necessary for wild type shiny and faceted antennae, indicating that the sand is turned into TWO final products (one substrate is used by two different enzymes). Feeding chemicals L and M (together, but not separately) to all mutant meeps will restore their wild type antennal bobble appearance. Sometimes, feeding them single intermediates (L-P) will also restore aspects of antenna appearance. Using the information in the table below, in which we feed the mutant meeps from the previous question the various chemicals, draw out the meep biochemical pathway and identify which steps are broken in which mutant meeps.
Meep mutations A, B, and C: dull and nonfaceted
Meep mutations D, E, and F: dull, faceted antennae
Meep mutations G, H, and I: shiny, nonfaceted antennae
Use the information in the chart to deduce the meep antenna bobble pathway. It is a branching pathway that begins with sand. Identify the order of intermediates (L-P) produced at each step in the circles, and which of the mutant lines affects which steps within the pathway above the arrows.
Considering both the complementation test data and the data from this chart, which enzyme is a potential heterodimer? Explain your answer in a sentence.
dull non shin fac dull non dull shin fac dull non shin fac shin fac shin fac dull fac shin fac shin fac shin fac dull fac dull fac shin fac shin fac shin fac dull fac dull fac dull fac shin fac shin fac shin fac shin fac shin fac shin fac shin non shin fac shin fac shin fac shin fac shin fac shin fac shin fac shin non shin fac shin fac shin fac shin fac shin fac shin fac shin fac shin fac shin non non Dull non-dull, nonfaceted; dull fac-dull, faceted; shin fac-shiny, faceted; shin non-shiny,Explanation / Answer
The following are represent a separate complementation groups:
So, there are total 7 complementation groups (Answer ‘1’ and ‘2’)
Answer 3.
A complementation group represents two mutants which are same. ‘A’ and ‘C’ are present in the same complementation group. They are the same mutants. So, a cross between them gives the same results.
By definition, a complementation group is a group of mutant genes which do not complement each other to produce a new phenotype.
**Kindly post rest of the parts separately.