Microbiology Chapter 6. Microbial nutrition and growth Review for the exam. Plea
ID: 202076 • Letter: M
Question
Microbiology Chapter 6. Microbial nutrition and growthReview for the exam. Please type for me and explain the concept that I should prepare for the exam. C6.22 Typical microbial growth curve Growth rate vs Death rate Compare Lag phase vs Stationary phase C6.23 Why do you need continuous culturing? Application in Biotechnology Chemostat C6.24 Measuring Microbial Reproduction Direct vs Indirect Methods Requiring vs Not Requiring Incubation Chapter 6 FACTS F6.1 Microbial Growth Requirements Chemicals Four basic groups of organisms based on their carbon and energy sources F6.2 Groups of organisms based on oxygen requirement F6.3 Oxygen requirements due to presence Or absence of certain enzymes F6.4 Nitrogen requirements Nitrogen fixation F6.5 Four categories of microbes based on temperature ranges for growth. F6.6 Microbial Growth Requirements Physical pH F6.7 Microbial Growth Requirements Water: Physical Requirement Hydrostatic pressure
Explanation / Answer
I have explained the topic related to the microbial growth and nutrition that may be helpful for your exam
1. Bacterial Growth Curve
The growth of bacteria in a fresh medium is divided into four phase.
A) Lag phase: During this phase, the bacteria will try to adapt to growth conditions. Also the maturation of bacteria will takes place, however the cell division wont happen.
B) Log phase: This is also called logarithmic phase or the exponential phase. The doubling of cells at the constant rate will occur during this phase.
C) Stationary phase: The growth and death rates of cell will be equal during this phase as the result of nutrient depletion and acid production.
D) Death Phase: In this phase, the bacteria will die due to the lack of nutrients and environmental temperature that is above or below the tolerance band for the species.
2. Measurement of microbial reproduction
The methods for measuring microbial growth are
A. Plate Counts which is based on the number of variable cells. However, it is time consuming as its takes a large portion of time for colonies to form.
B. Serial Dilutions
C. Pour Plates and Spread Plates-based method that uses nutrient agar as a medium for the growth of colonies.
3. Chemostat
It is a type of bioreactor, into which the fresh medium are added continuously, while removing the culture medium containing left over nutrients, metabolic end products, and microorganism