Part A The addition of a \"5-cap\" and a \"3\' poly-A\" tail to many nuclear RNA
ID: 202864 • Letter: P
Question
Part A The addition of a "5-cap" and a "3' poly-A" tail to many nuclear RNA species occurs prior to export of mature mRNAs to the cytoplasm. Such modifications appear to influence mRNA stability. Many assays for gene regulation involve the use of reporter genes such as luciferase from the North American firefly (Photinus pyralis). When the luciferase gene is transcribed and translated, the protein product can be easily assayed in a test tube. In the presence of luciferin ATP, and oxygen, the luciferase enzyme produces light that can be easily quantified. Assuming that luciferase mRNA can be obtained and differentially modified (5'-capped, poly-A tailed) in a test tube, suggest an assay system that would allow you to determine the influence of 5'-capping and poly-A tail addition on mRNA stability. One could test a number of different constructs (comparing normal protein with enzymes of 5-capped, poly-A tailed, and with mutated enzymes of 5- capped, poly-A tailed) and test for luciferase synthesis in the assay system described in the problem Just one test of normal protein and enzyme of 5-capped and poly-A tailed for luciferase synthesis in the assay system described in the problem will be enought for determinition of stability One could test a number of different constructs (comparing normal protein with enzymes of 5-capped, poly-A tailed, and with mutated enzymes of 5- capped, poly-A tailed) and test for luciferase activity in the assay system described in the problem One could test a number of different constructs (comparing normal protein with enzymes of 5-capped, poly-A tailed, and mRNA without both additions) for luciferase activity in the assay system described in the problem. Submit uest AnswerExplanation / Answer
If we want to test whether there is some effect of mRNA capping and poly A tail then we need to use two sets of the experiment. One will have all the enzyme for capping and polyA tail and another one will not have capping and polyA tail enzymes and check for luciferase activity.
So correct answer is option C.