Please answer the following questions using the figure above: 1. What is the seq

Question

Please answer the following questions using the figure above:

1. What is the sequence of primer 'a' (include prime ends directionality (i.e. 5’ vs 3’)?

2. What is the sequence of primer 'c' (include prime ends directionality (i.e. 5’ vs 3’)?

3.Which primer could be used together with primer 'c' to amplify a portion of the DNA sequence?

4.How many base pairs long would the product be from a PCR amplification using primers 'a' and 'b'?

5.Would an amplicon result from the use of primer ‘a’ and ‘d’? Why or why not?

Arrow Notation for DNA DNA Sequence 5-CATGCCGTGGAATGGGAACTCGAGGCGCGGA ATGAGGATGCTAACTATACACATATTAATGGIATTAACACGGAGCGTGR GAGTACTATAAACGGCTACGAAACG-3 3'-GTACGGCACCTTACCCTTGAGCTCCGCGCCT TACTCCTACGATTGATATGTGTATAATTACCATAATTGTGCCACGCACICTCATGATATTTGCCGATGCTTTGC5 A single strand of DNA Double-stranded DNA Oligonucleotides (primers) Primers bound to DNA strands

Explanation / Answer

answers are provided in a sequential mannner below:

A1) 5- tggaatgggaact -3

A2) 5- ccgtgttaatacca -3

A3) Primer A can be used as forward primer against primer C

A4) 37 base pairs

A5) No, there would be no amplicon as the amplification would result in only single direction with both primers. There is no reverse primer

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