Please Answer ALL questions. Thanks! 11) The genetic code is universal, as are t
ID: 204983 • Letter: P
Question
Please Answer ALL questions. Thanks!
11) The genetic code is universal, as are the core genetics processes of transcription and translation, yet there are subtle differences in Eukaryote and Bacterial transcription and translation. You have been asked to engineer a bacterial gene that conveys resistance to heavy metals (BVR 1) into the Zea mays (corn). What modifications would you need to make to the gene in order for it to be transcribed and translated by the plant? 12) A mutation is defined as a change in the sequence of a DNA molecule. Sometimes mutations cause no obvious changes, but at other times mutations can have profound effects on the synthesis or t function of a protein. The nucleotide sequences of the DNA molecules below are the same except for a single point mutation (single nucleotide polymorphism). The first nucleotide in the DNA sequence is the start point of transcription. Using the table provided, transcribe and translate each of the sequences, then answer the questions below Normal DNA Direction of RNA Polymerase lI Second letter Sequence 5'-GTACGAACGCGAGTTCGTATATAGCAGCAT-3" UCU UCC UGU UGC Ul 3'-CATGCTTGCGCTCAAGCATATATCGTCGTA-5' UAA Stop UGA Stop A UAG Stop UGG Trp G CGU CGC CGA Arg Ccu Transcribe CCA CCG Translate: AUC lle ACC Mutatio 5'-GTACTAACGCGAGTTCGTATATAGCAGCAT-3' 3'-CATGATTGCGCTCAAGCATATATCGTCGTA-5' AUG Met ACG GUU GUC GUA Val GCC GGU GGC GUG GCG Transcribe: Translate: What do you think the effect of the mutation will be?Explanation / Answer
>Normal DNA
GTA CGA ACG CGA GTT CGT ATA TAG CAG CAT - DNA
CAU GCU UGC GCU CAA GCA UAU AUC GUC GUA - mRNA
1 AUG CUG CUA UAU ACG AAC UCG CGU UCG UAC 30
1 Met Leu Leu Tyr Thr Asn Ser Arg Ser Tyr 10 - Polypeptide
>Mutation 1
GTA CTA ACG CGA GTT CGT ATA TAG CAG CAT - DNA
CAU GAU UGC GCU CAA GCA UAU AUC GUC GUA - mRNA
1 AUG CUG CUA UAU ACG AAC UCG CGU UAG UAC 30
1 Met Leu Leu Tyr Thr Asn Ser Arg End Tyr 10 - Polypeptide
The UCG changes UAG. Normal amino acid codon to termination codon. Hence it is an example for nonsense muation.