ID: A Multiple Choice Mdentify the letter of the choice thar best completes the
ID: 205318 • Letter: I
Question
ID: A Multiple Choice Mdentify the letter of the choice thar best completes the statement or amsers 17. A nevw is an example of a product that could have been derived from genetic en b. vitamin e. metal d. centrifuge -18. Before recombinant cells are produced, what must be done? a. An assay must be done to confirm protein activity in transformed cells The recombinant protein is isolated, purified, and formulated. The DNA encoding for the desired characteristic is identified, isolated, and confirmed. b c- d. Fermentation must be optimized. 19. What treatment encourages cells to incorporate rDNA plasmids during a bacterial transformation _ a. Heat shock in warm water bath followed by ice bath b. Plating on nutrient agar with antibiotic e. Achieving exponential cell growth d. Assaying for recombinant proteins Rennin was used in cheese making, but due to its inconsistent availability, was a. 20. replaced by its genetically engineered equivalent, Chymosin. hybridized with another protease. lysed and precipitated out of solution. b. c. d. All of the above What is not a form of selection used to identify transformed cells? a. 21. The green fluorescent protein gene is included on the vector plasmid. b. Suspected transformed cells are spun in a centrifuge. c. An antibiotic resistance gene is added to the vector. d. The target gene is recombined into a plasmid with the beta-galactosidase gene. An example of a DNA vector is a(n) a. ribosome b. fungus. c. algae. d. plasmid. A piece of DNA that results from being spliced into a vector is called a. a sticky end. b. recombinant DNA. c. an endonuclease. d. an RFLP 22. 23. 24. What is the purpose of adding cations (Ca or Mg) to host cells before a transformation? a. b. c. d. Increases transformation efficiency Allows for selection of transformed cells Gets rid of sticky ends All of the aboveExplanation / Answer
17. In general new proteins such as antibodies, thrombolytics, growth factors, interleukins etc derived from the genetic engineering, hence, correct answer is pharmaceuticals is correct and more correct is bio-pharmaceutical.
18. before producing any protein DNA sequence should be checked than only production will start. Correct answer is C
19. Heat shock and cold shock open and close the pores of cell membrane that facilitate the entry if recombinant DNA. Correct answer is A.
20.Chymosin is an enzyme that produced recombinantly and used for cheese making. Correct answer is a.
21. Correct answer is b. transformed and non-transformed cells cannot be detected by spin.
22.Plasmid is a DNA vector.
23.Recombinant DNA.
24. Ca and Mg ions bind with DNA and these ion bind with cells, therefore, they increase the efficiency of DNA.