ID: A Name: 15. A few high-risk patients are used to help determine drug safety,
ID: 214292 • Letter: I
Question
ID: A Name: 15. A few high-risk patients are used to help determine drug safety, dosage, and potential side effets Several hundred patients are used to study safety, dosage, and efficacy of the product. -17. Lar ge and diverse populations are used to better understand the safety and efficacy of the new drug. For the following items, decide which gel filtration characteristic (a-c) should be altered to optimize the a. column length b. column width c. pore size 18. Especially large molecules must be separated 19. A large batch of proteins from the largest fermentation tank needs to be isolated 20. Proteins of similar size need to be separated Multiple Choice ldentify the letter of the choice that best completes the statement or answers the question 21. What is the purpose of SDS when harvesting intracellular proteins? a. It solubilizes extracellalar proteins, making it easier to recover the intracellular ones b. It is used during sonication. c. It degrades the cell membrane causing the cells to burst. d. All of the above proteins are easier to isolate from cell cultures. a. Extracellular b. Intracellular c. Transcellular d. Antigenic What is the purpose of conducting double-blind tests during clinical trials? a. 23. To minimize the chance a patient receives a placebo b. To prevent cross-contamination of product c. To help keep track of the numerous patients involved in the trial d. To help ensure that neither the experimenter nor the test subject can influence the results All of the following might be reasons a product would not be marketable except: -24. Patent protection may not be secured a. b. Assays 99.9% reactivity c. Production of the product is uneconomical d. FDA may not approve the product 25. How might the fractions containing colorless target proteins be visualized after chromatography? a. DPIP testing b. agarose gel electrophoresis c. PAGE gel EtBr dot testing d.Explanation / Answer
18. The answer is b.
Explanation
The larger particles pass through the spaces in between the pores. So, as the column width increases, the larger molecules must be separated.
19. The answer is a.
Explanation
As the column length increases, the more space creates and so the large batch of proteins can be separated.
20. The answer is c.
Explanation
The smaller sized proteins move through the pores, they elute later i.e. after larger molecules. Thus, if the pore size increases, the smaller proteins need to be separated.